Molecular diagnostics

Polymerase Chain Reaction (PCR):
Most molecular pathological tests performed focus on somatic or acquired DNA variations in the cells of the disease process that provide information that aids in diagnosis, identifies prognostic indicators, stratifies patients into effective treatment options, helps monitor treatment response, and identifies patients at increased risk of disease. Because polymerase chain reaction (PCR)-based approaches are quick, reliable, and sensitive, PCR (e.g. K-ras and BRAF mutation) has become a central technology for much of clinical molecular genetic testing.


Florescence in situ hybridization:
Florescence in situ hybridization (FISH) uses tagged probes that bind to chromosome-specific DNA sequences of interest, thereby allowing for the identification of both structural and numeric aberrations that specify certain (non-)hematopoietic malignancies. It has several major advantages over conventional cytogenetics in that it can be applied in many clinical settings, can be performed on non-dividing cells, can be performed on air-dried or formalin-fixed specimens, can facilitate detection of molecular abnormalities in neoplasms with low proliferation rates (e.g. myeloma), and can facilitate detection of numeric abnormalities. In surgical pathology, this technique is used primarily to detect somatic cancer-associated alterations for diagnostic, prognostic, or therapeutic purposes.

Our institute performs Her2/Neu FISH routinely and K-ras and EGFR selectively.
 

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